Binding diversity of monoclonal antibodies toα(2 → 8) polysialic acid conjugated to outer membrane vesicle via adipic acid dihydrazide
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چکیده
منابع مشابه
Preparation of enzyme conjugate through adipic acid dihydrazide as linker and its use in immunoassays.
The direct coupling of the carboxylic derivative of steroids to the amino group of enzymes is a well-established method in steroid enzyme immunoassays for making enzyme conjugates (1). Horseradish peroxidase (HRP), containing six lysine residues in the sequence, is a widely used enzyme in enzyme immunoassays; in practice, however , only one or two of these are generally available for reaction (...
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BACKGROUND Biobased processes for the production of adipic acid are of great interest to replace the current environmentally detrimental petrochemical production route. No efficient natural producer of adipic acid has yet been identified, but several approaches for pathway engineering have been established. Research has demonstrated that the microbial production of adipic acid is possible, but ...
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Hybridoma-derived monoclonal antibodies were prepared against outer membrane antigens of four strains of Vibrio cholerae that were cultivated under iron-limited conditions, and these antibodies were partially characterized. We established a library of 66 hybridomas which produced monoclonal antibodies defining 16 different V. cholerae antigens. Two antigens (molecular weights, 18,000 and 112,00...
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Five monoclonal antibodies that recognize chicken brain glutamic acid decarboxylase (GAD) have been selected and designated GAD-1 to -5. GAD-1 to -5 were selected on the basis of their ability to immunoprecipitate active GAD from crude brain extracts. GAD-1 recognizes an epitope that is conserved in many vertebrates; the epitope recognized by GAD-5 is restricted to the chicken. Radioimmunoassay...
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CCN activation experiments with adipic acid: effect of particle phase and adipic acid coatings on soluble and insoluble particles S. S. Hings, W. C. Wrobel, E. S. Cross, D. R. Worsnop, P. Davidovits, and T. B. Onasch Department of Chemistry, Boston College, Chestnut Hill, MA 02467, USA Aerodyne Research Inc., Billerica, MA 01821, USA Received: 1 February 2008 – Accepted: 1 February 2008 – Publi...
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ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 1996
ISSN: 0928-8244,1574-695X
DOI: 10.1111/j.1574-695x.1996.tb00289.x